It's really over.

Hugh Hefner confirms he and Holly Madison are no longer.

"If she says it's over, it's over," Hefner tells me. "But like I've said before, she is the love of my life, and I expected to spend the rest of my life with her."

The Playboy mogul says the relationship started to crumble about six months ago when they learned he was unable to father a child with Holly.

"With my sperm count, it's not possible," Hefner explained. "I was willing but it was not possible…She'd like to be married and have children, but it's not in the cards here for me."

Where does that leave the other two Girls Next Door, Kendra Wilkinson and Bridget Marquardt?

Hefner says they're still romantically involved, but admitted, "the relationship with Kendra will be ending when she moves out, probably by the end of this year."

Hef ain't wasting anytime getting back in the saddle though.

In the last week, he's been spotted with 19-year-old twins Kristina and Karissa Shannon (pictured above). "They very much want to be girlfriends and now under the present circumstance, they probably will become my girlfriends," Hefner says.

And they're not all he has to look forward to.

A college senior from Georgia whom Hefner identifies as Amy Leigh Andrews is also waiting in the wings. "She was testing here this past weekend," Hef says of the centerfold wannabe, "and she said she'd like to be a girlfriend, too."

The 82-year-old Hefner laughed, "I'm dancing as fast as I can."

heres another pic of his new girlfriends

HERES A PICTURE OF THE TWINS)
http://images.eonline.com/eol_images...

HERES A PIC OF AMY LEIGH

http://www.galleries.badgirlsblog.co...

Im sorry but they are gold diggers! They look it to!
Hef's lowerd his standards!

oxox Chloe

UPDATE******

Very sad note: Prior to making his MySpace private today, the father of the Shannon twins was bragging about his daughters sleeping/going out with Hef . Sick.

Hef and I care about each other immensely and will always be best friends . . . I do have my own place, but I am still at the Mansion, too, right now . . . I'm too busy to move even if I wanted to! hahaha . . . Bridget, Kendra and I are all still best friends and plan on doing several projects together in the future . . . even though Bridget is in Europe right now and I am sad and lonely without her . . .

You will see how it all happens in Season 5 . . . Sunday nights on E! . . . How lame, I just turned that into the most shameless plug ever . . . lol!

Love-Holly

For the past few weeks there have been many rumors about the "Girls Next Door" falling apart, moving out of the mansion and being replaced by younger models. There has also been a little rumor that Holly Madison is doing it with a magician who goes by the name of Criss Angel. Hef and Holly both denied all the rumors.

Last night, a TMZ camera man chatted with Holly outside of a restaurant and he told her he wanted to go to the Playboy Halloween party and if she could get him in. Holly yammered on about how she was going to be Elvis for Halloween and then she said, "I have no pull anymore. Hef and I aren't together"

Holly also said that she's still filming shit with Kendra and Bridget for the sixth season of "GND."

Heres the video Click on the link

http://www.tmz.com/videos?autoplay=t...